谢菲尔德哈勒姆大学PhD position in Multimodal mass spectrometry imaging to visualise the glycomic and metabolomic landscape for prostate cancer metastasis to bone申请条件要求-申请方
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谢菲尔德哈勒姆大学
PhD直招介绍
About the Project
Prostate cancer frequently metastasises to bone, causing considerable morbidity and mortality. Once in the bone, prostate cancer cells disrupt normal bone turnover leading to clinically osteosclerotic lesion with underlying osteolysis. Tumour cells exhibit altered post-translational modification of many proteins, which affect tumour cell behaviours including invasion and metastasis. Specifically, N-glycans are a group of post-translational modification that are altered in prostate cancers and affect bone metastasis. A key enzyme in the formation of mature N-glycosylations is Fucosyltransferase 8 (Fut8). Fut8 is overexpressed in prostate cancers and has been strongly linked to bone metastasis.
The detection and imaging of N-glycans by mass spectrometry imaging is in its infancy and this cutting-edge technique has been recently established in Sheffield. We will use two models of prostate cancer grown in vivo and in advanced cell culture models.
We aim to:
Assess whether current methods of tissue processing of in vivo tissues affect N-glycan and metabolite detection by mass spectrometry imaging
Compare glycomic and metabolomic landscape of cells grown in 2D cell culture vs 3D cell co-culture with bone marrow stromal cells vs in vivo bone metastasis,
Assess the impact of N-glycosylation-modifying drugs (including Fut8 inhibitors) on N-glycans in vivo vs 3D cell culture and d) assess the impact of FUT8 knockdown on N-glycans in vivo vs 3D cell culture.
Detection of glycans and metabolites will be performed by Desorption Electrospray Ionisation Mass spectrometry imaging (DESI-MSI) and/or Matrix-assisted Laser-desorption Ionisation Mass spectrometry imaging (MALDI-MSI). Glycan detection on tissues will be detected following Peptide N-glycosidase F (PNGase) or EndoF3 digestion of tissues prior to MSI detection of cleaved glycans. The glycomic and metabolomic landscape of in vivo tissues generated from a FUT8 knock-down PC3 cell line grown in bone, along with syngeneic prostate cancer cells grown in bone will be used and compared with a FUT8 inhibitor, along with comparisons of in vivo prostate cancer cells in bone with 3D cell culture of prostate cancer/bone marrow stromal cell co-cultures.
About YBDTP:
YBDTP brings together world-class bioscience research and innovation, as well as excellence in doctoral supervision, across the region. YBDTP will fund postgraduate researchers at the Universities of Leeds, Sheffield, York, Bradford, Hull, Leeds Beckett, Sheffield Hallam and Teesside forming a strong regional training partnership. In YBDTP you'll benefit from a regional doctoral training programme that has interdisciplinary collaboration at its core. The aim is to enable you to develop a range of research skills in biological, biotechnology and biochemical areas as well as equip you with core data analysis and professional skills that are necessary for bioscience research and related non-academic careers.
https://www.whiterose-mechanisticbiology-dtp.ac.uk/yorkshire-bioscience-dtp/
谢菲尔德哈勒姆大学 PhD position in Multimodal mass spectrometry imaging to visualise the glycomic and metabolomic landscape for prostate cancer metastasis to bone项目有没有奖学金，是不是全奖Phd招生，下面我们一起看一下【大学名称】Phd的奖学金资助情况
项目资助情况
A tax-free annual stipend at the standard UKRI rate (£19,237 for 2024/25 entry), research costs and tuition fees at UK rate
Academic year: 2025/26
Open to: International (including EU) and UK (home) students
The studentships are fully funded for four years, and you must complete your PhD in four years.
You'll receive the UKRI minimum doctoral stipend per year for your living costs, which is paid to you in regular instalments. The UK Research and Innovation( https://www.ukri.org/apply-for-funding/studentships-and-doctoral-training/get-a-studentship-to-fund-your-doctorate/ ) (UKRI) stipend is tax free and does not need to be returned.
费用
International students will need to have sufficient funds to cover the costs of their student visa( https://www.gov.uk/student-visa ), NHS health surcharge( https://www.gov.uk/healthcare-immigration-application/how-much-pay ), travel insurance and travel to the UK as these are excluded from UKRI funding.
谢菲尔德哈勒姆大学Phd申请条件和要求都有哪些？PhD position in Multimodal mass spectrometry imaging to visualise the glycomic and metabolomic landscape for prostate cancer metastasis to bone项目是不是全奖？有没有奖学金？下面我们一起看一下谢菲尔德哈勒姆大学申请Phd直招需要具备哪些条件和要求，以及托福、雅思语言成绩要到多少才能申请。
申请要求
Open to International (including EU) and UK (home) students.
You can apply if you have, or are expecting to gain, at least an upper second-class honours degree or equivalent. Due to the interdisciplinary nature of this programme, we welcome applications if your background is in any biological, chemical or physical science or mathematics and are interested in using your skills in addressing biological questions. International students should check if they meet the entry requirements for their country( https://www.york.ac.uk/study/international/your-country/ ).
We aim to support the most outstanding applicants from outside the UK and are able to offer a limited number of bursaries that will enable full studentships to be awarded to international (EU and non-EU) applicants. These full studentships will only be awarded to exceptional quality candidates, due to the competitive nature of this scheme.
YBDTP is committed to recruiting extraordinary future scientists regardless of age, ethnicity, gender, gender identity, disability, sexual orientation or career pathway to date. We understand that commitment and excellence can be shown in many ways and have built our recruitment process to reflect this. We welcome applicants from all backgrounds, particularly those underrepresented in science, who have curiosity, creativity and a drive to learn new skills.
Not all projects will be funded; a limited number of candidates will be appointed via a competitive process.
English language:
If English isn't your first language, you may need to provide evidence of your English language ability.
报名方式
申请链接
联系人
邮箱：wrdtp@leeds.ac.uk
招生人信息
Dr Neil Cross
邮箱：N.Cross@shu.ac.uk
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